Mitochondria Research

Mtphagy Dye (included in Mitophagy Detection Kit) is a pH-sensitive fluorescent probe that accumulates in mitochondria and emits red fluorescence due to acidic conditions in a lysosome.

A pH-sensitive ratiometric fluorescent protein. The excitation spectrum changes according to pH. This protein shows high fluorescence ratio (Ex. 550 nm/440 nm) values in a lysosome.

Yes
（Covalent bond）

-
（Expression protein）

Live-cell staining
（Fixation after live-cell staining）

✓
(-)

✓
(-)

Fixed cell staining

Ex/Em (nm)

Staining time^*1

30 min～

Product code

Superoxide

A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with lipophilic peroxides in mitochondria to emit fluorescence.

A cell-permeant fluorescent probe that  reacts with ROS  generated in mitochondria to emit fluorescence.

A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with singlet oxigen generated in mitochondria to emit fluorescence.

A cell-permeant fluorescent probe that accumulates in mitochondria and reacts with superoxide generated in mitochondria to emit fluorescence.

A cell-permeant fluorescent probe that accumulates in mitochondria and reacts with superoxide generated in mitochondria to emit fluorescence.

Yes
（Electrostatic interaction）

Yes
（Electrostatic interaction）

Yes
（Electrostatic interaction）

Yes
（Electrostatic interaction）

✓
(-)

✓
(-)

✓
(-)

✓
(-)

✓
(-)

TexasRed

Cy5

Cell-permeant fluorescent dyes accumulate in intact mitochondria due to the membrane potential. MT-1 is extremely photostable and more sensitive than JC-1 and can provide equivalent detection sensitivity to TMRE.

A cell-permeant ratiometric fluorescent dye that accumulates in intact mitochondria due to the membrane potential. JC-1 forms aggregate (in healthy mitochondria) with red fluorescence.

As membrane potential decreases, JC-1 becomes monomers, which shows in green fluorescence.

TRITC

TexasRed

Cell-permeant fluorescent dyes accumulate in intact mitochondria due to the membrane potential.

Cell-permeant fluorescent dyes accumulate in intact mitochondria due to the membrane potential.

Cell-permeant fluorescent dyes accumulate in intact mitochondria due to the membrane potential.

Cell-permeant fluorescent dyes accumulate in intact mitochondria due to the membrane potential.

TRITC

-

FITC

A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with calcium ion in mitochondria to emit red fluorescence.

A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with ferrous ion in mitochondria to emit green fluorescence.