検出対象 |
バイオレセプター |
トランスデューサー |
使用試薬 |
参考論文 |
複数の生体分子 |
DNA/Protein Microarrays |
SPR imaging |
AB-NTA |
T. Seefeld, A. Halpern, and R. Corn, On-Chip Synthesis of Protein Microarrays from DNA Microarrays Via Coupled In Vitro Transcription and Translation for Surface Plasmon Resonance Imaging Biosensor Applications, J. Am. Chem. Soc., 2012, 134(30), 12358. |
特定タンパク質 |
RNA aptamer |
SPR imaging |
11-Amino-1-undecanethiol, hydrochloride |
Y. Li, H. Lee, and R. Corn, Fabrication and characterization of RNA aptamer microarrays for the study of protein–aptamer interactions with SPR imaging. Nucleic Acids Res. 2006, 34, 6416. |
anti-SARS-CoV-2抗体 |
蛍光標識抗体 |
蛍光偏光イムノアッセイ(FPIA) |
HiLyte Fluor™ 647 Labeling Kit-NH2 |
K. Nishiyama, K. Takahashi, M. Fukuyama, M. Kasuya, A. Imai, T. Usukura, N. Maishi, M. Maeki, A. Ishida, H. Tani, K. Hida, K. Shigemura, A. Hibara, and M. Tokeshi, Biosens Bioelectron, Facile and rapid detection of SARS-CoV-2 antibody based on a noncompetitive fluorescence polarization immunoassay in human serum samples., Biosens Bioelectron., 2021, 190, 113414. |
ヒトパピローマウイルス(HPV) |
POD標識抗体
Biotin標識抗体 |
ELISA |
Peroxidase Labeling Kit - NH2
Biotin Labeling Kit - NH2 |
R. Hu, Z. Dong, K. Zhang, G. Pan, C. Li, and H. Cui, Preparation, Characterization and Diagnostic Valuation of Two Novel Anti-HPV16 E7 Oncoprotein Monoclonal Antibodies, Viruses, 2020, 12, 333. |
血清クレアチニン |
HRP標識抗体 |
gold electrode chips |
Biotin Labeling Kit - NH2 |
F. Wei, S. Cheng, Y. Korin, E. Reed, D. Gjertson, C. Ho, H. Gritsch, and J. Veale, Serum Creatinine Detection by a Conducting Polymer Based Electrochemical Sensor to Identify Allograft Dysfunction, Anal. Chem., 2012, 84, 7933. |
血糖値
(2.5世代グルコースセンサー)
|
PES修飾FAD-GDH |
電極 |
Amine-reactive PES |
M. Hatada, N. Loew, Y. Inose-Takahashi, J. Okuda-Shimazaki, W. Tsugawa, A. Mulchandani, K. Sode, Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference, Bioelectrochemistry, 2018, 121, 185. |
血糖値
(2.5世代グルコースセンサー) |
PES修飾GOx |
電極 |
Amine-reactive PES |
N. Suzuki, J. Lee, N. Loew, Y. Takahashi-Inose, J. Okuda-Shimazaki, K. Kojima, K. Mori, W. Tsugawa and K. Sode, Engineered Glucose Oxidase Capable of Quasi-Direct Electron Transfer after a Quick-and-Easy Modification with a Mediator, Int. J. Mol. Sci., 2020, 21, 1137. |
乳酸 |
PES修飾 L-lactate oxidase (LOx) |
電極 |
Amine-reactive PES
※アスコルビン酸、アセトアミノフェンの添加影響を確認 |
K. Hiraka, K. Kojima, W. Tsugawa, R. Asano, K. Ikebukuro, and K. Sode, Rational engineering of Aerococcus viridans l-lactate oxidase for the mediator modification to achieve quasi-direct electron transfer type lactate sensor, Biosensors and Bioelectronics, 2020, 151, 111974. |
HbA1c |
PES修飾fructosyl peptide oxidase (FPOx) |
フローインジェクション分析 |
Amine-reactive PES |
M. Hatada, S. Saito, S. Yonehara, W. Tsugawa, R. Asano, K. Ikebukuro, and K. Sode, Development of glycated peptide enzyme sensor based flow injection analysis system for haemoglobin A1c monitoring using quasi-direct electron transfer type engineered fructosyl peptide oxidase. Biosensors and Bioelectronics, 2021, 177, 112984. |
L-glutamine |
PES修飾L-glutamine-binding protein (QBP) |
電極 |
Amine-reactive PES |
S. Takamatsu, J. Lee, R. Asano, W. Tsugawa, K. Ikebukuro, and K. Sode, Continuous electrochemical monitoring of L-glutamine using redox-probe-modified L-glutamine-binding protein based on intermittent pulse amperometry, Sensors and Actuators B: Chemical, 2021, 346, 130554. |